Official publication of Rawalpindi Medical University
Effect of chronic restraint stress on body weight of male Sprague Dawley rats

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Body weight, Chronic stress, cortisol

How to Cite

Moazzam S, Mughal IA, Irfan NUA, Attique I, Zaheer F, Irfan A. Effect of chronic restraint stress on body weight of male Sprague Dawley rats. JRMC [Internet]. 2023 Apr. 2 [cited 2024 May 26];27(1). Available from:



Stress disturbs body weight and food intake, but the core mechanisms are not well understood. 


Study design:  Experimental  


Materials and Methods: 

The study was conducted in  National  Institute  of  Health  (NIH),  Islamabad Sixty male Sprague Dawley rats (mean wt 250 ± 50 grams) were used and divided into 2 groups.  

Each group comprised of 30 rats.  

Group I was taken as control. Group II was exposed to chronic stress. 


The comparison of weight gain by the different groups disclosed that there was a significant decrease in weight gain of rats exposed to the chronic stress as compared to control group.  



Chronic restraint stress has a detrimental effect on body weight.


Ablimit A, Kühnel H, Strasser A, Upur H. Abnormal Savda syndrome: long-term consequences of emotional and physical stress on endocrine and immune activities in an animal model. Chin J Integr Med. 2013;19(8):603–609..

Smith AP. Effects of upper respiratory tract illnesses and stress on alertness and reaction time. Psychoneuroendocrinology. 2013;38(10):2003–2009.

Srinivasan S, Loganathan S, Wankhar W,Rathinasamy S, Rajan R. Stress effect on humoral and cell mediated immune response: Indispensible part of corticosterone and cytokine in neutrophil function.Trials in vaccinology 2016; 5: 61-70.

Loveless SE, Hoban D, Sykes G, Frame SR, Everds NE. Evaluation of the immune system in rats and mice administered Linear Amminium Perfluorooctanoale.Toxicological Sc 2008 ;105(1): 86- 96.

Belay T, Woart A. Cold-induced stress increases the intensity of Chlamydia genital infection in mice. J Microbiol Immunol Infect. 2017;46(5):330–337

Zieziulewicz TJ, Mondal TK, Gao D, Lawrence DA.Stress-induced effects, which inhibit host defenses,alter leukocyte trafficking. Cell Stress Chaperones. 2013;18(3):279–291

Sarjan HN, Yajurvedi HN. Chronic stress induced duration dependent alteration in immune system. Immunology Letters 2018;75:1357–1368

Liu N, Wang LH, Guo LL, Wang GQ, Zhou XP, Jiang. Y et al. Chronic restraint stress inhibits hair growth via Substance P mediated by Reactive Oxygen species in mice. PLOS ONE 2013; 8(4); e61574

HuGZ,YangSJ,HuWX,WenZ,HuD,ZengLF, Xiang Q, Wu XM et.,al. Effect of cold stresson immunity in rats. Exp Ther Med 2016; 11(1):33- 42.

Everds NE, Snydes PW, Bailay KL, Bolon B, Creasy DM. Intepreting stress responses during routine toxicity studies: A review of the Biology, Impact and assessment. Toxicologic Pathology2013; 41(4): 561- 614.

Marti O, Marti J, Armario A. Effects of chronic stress on food intake in rats:influence of stressor intensity. and duration of daily exposure. Physiol Behav. 1994; 55(4):747-53.

Harris RB, Zhou J, Youngblood BD, Rybkin II, Smagi GN, Ryan DH. Effect of repeated stress on body weight and body composition of rats fed low- andhigh- fat diets. Am J Physiol 1998;275(6): 1928-1938.

Gamaro GD, Manoli LP, Torres IL, Silveira R, Dalmaz C. Effects of chronic variate stress on feeding behavior and on monoamine levels in different rat brain structures. Neurochem Int. 2003;42(2):107- 14.

Santos J, Benjamin M, Yang PC, Prior T, Perdue MH. Chronic stress impairs rat growth and jejunal epithelial barrier function: role of mast cells. Am J Physiol Gastointest Liver Physiol 2000; 278(6): 847-

Dallman MF, Pecoraro N, Akana SF, , Fleur SEI, Gomaz F, Houshyar H . Chronic stress and obesity: a new view of Comfort food. Proc Natl Acad Sci USA 2003; 100(20): 11696- 701

Fuenta MDI, Ferrandoz MD, Burgos MS, Solar A, Prieto A, Miquel J. Immune function in aged women is improved by ingestion of Vitamin C and E. Can J Physiol Pharmacol. 1998; 76(4); 373-80.

Ark PC, Slominski A, TheoharidesTC, Peters EM, Paus R. Neuroimmunology of stress: skin takes centre stage. J Invest Dermatol 2006; 126(8): 1697- 1704.

KAB Moien, Menon P, GovenderR, Samra AMBA, Allaham KK, Nauman J.Systematic review of the effects of pandemic confinements on body weight and their determinants British Journal of Nutrition (2022), 127, 298–317

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Copyright (c) 2023 Sadia Moazzam, Irfan Afzal Mughal, Noor-ul-Ain Irfan, Ilqa Attique, Fareeha Zaheer, Asma Irfan